Affiliation

Postdoc
Department of Animal and Veterinary Sciences

MOVE host lab

Prof. Alireza Fazeli and Dr. Suranga Kodithuwakku
University of Tartu, Institute of Biomedicine and Translational Medicine
Estonian University of Life Sciences, Institute of Veterinary Medicine and Animal Sciences
Estonia

Grant amount and period of the research stay

DKK 15.000 for 2 months (Oct 2025 – Nov 2025)

EV techniques

EV isolation, upscaling and characterization from parasite vesicles

Project title

EV isolation and upscaling from helminths, host-cells and host feces

Project in a nutshell

EVs from Ascaridia galli are considered promising vaccine candidates, but their development is currently constrained by insufficient characterization and limited large-scale production. In this project, deep characterization and upscaling of A. galli EV production are pursued using combined differential centrifugation and size exclusion chromatography to improve EV yield, purity, and biological integrity. Isolation of EVs from fecal samples is also addressed, building on protocols developed by the host group and enabling analysis of parasite- and host-derived EVs under natural infection conditions. Access to the advanced EV isolation expertise and standardized methodologies available in Tartu is therefore considered essential to overcome current bottlenecks, enable antigen discovery, and support future comparative EV analyses across parasites and host systems.